UniqueKNOCK®
The UniqueKNOCK® kit is designed to overcome challenges associated with conventional transfection methods, such as low efficiency and high cytotoxicity. Specifically engineered for highly efficient and precise siRNA delivery and gene knockdown, it has evolved from a laboratory tool into a clinical-grade solution, providing a one-stop platform for gene silencing in scientific research and drug development. Using nano-needle delivery, siRNA is directly transported into the cytoplasm through nanoscale needle-like structures, effectively bypassing the toxicity of chemical transfection reagents and endosomal escape issues.
Long double-stranded RNA (dsRNA) is cleaved by the Dicer enzyme into small interfering RNAs (siRNAs). The siRNAs associate with the RISC complex, where the passenger strand is degraded and the guide strand directs RISC to recognize and cleave complementary mRNA. After cleavage, RISC can be recycled to continue targeting additional mRNA molecules, enabling sustained gene knockdown.
1、Regulation of primary immune cells, such as enhancing CAR-T or NK cells: Nano-needle delivery of siRNA enables knockout of immunosuppressive factors like PD-1 or SOCS1 in T cells, boosting tumor-killing activity.
2、Tumor microenvironment reprogramming, including immune cell engineering: Localized delivery of siRNA via nano-needle arrays knocks out TGF-β receptors in tumor-infiltrating lymphocytes (TILs), reversing the immunosuppressive microenvironment.
3、Gene knockdown in organotypic cultures of mouse gastric smooth muscle: siRNA is used in near-physiological in vitro models to investigate the regulatory mechanisms of specific genes on gastric smooth muscle functions, such as contraction, signal transduction, and pathological remodeling.
Mechanism of siRNA-Mediated Gene Knockdown
siRNA-Based Gene Knockdown in Organotypic Cultures of Mouse Gastric Smooth Muscle