UniquePOKE®
UniquePOKE® is an advanced nano-needle-based transfection kit designed to simplify and enhance gene delivery for both clinical and research applications. Utilizing vertically aligned nanostructures, UniquePOKE enables highly efficient, cost-effective, and biocompatible intracellular delivery of genetic material—without the need for viral vectors or chemical reagents. It provides a powerful solution for gene modification in therapeutic cell manufacturing, including immunotherapy and stem cell engineering, accelerating the commercialization of nano-needle biochip technology.
| Immortalized Cell Line | Primary Cells | |
|---|---|---|
| HEK293 | Jurkat | Neuron |
| A549 | RAW264.7 | Fibroblast |
| MCF7 | SH-SY5Y | Cardiomyocyte |
| HeLa | 3T3 | Mesenchymal stem cell |
| CHO | K562 | T cell |
1、Efficiently study the biological effects of target genes by transfecting overexpression plasmids, such as EGFP fusion vectors.
2、For primary cells (e.g., neurons, cardiomyocytes, stem cells, immune cells) and suspension cells that are difficult to transfect using conventional methods such as lipofection or electroporation, nano-needle transfection often demonstrates significant advantages, providing higher transfection efficiency and improved cell viability.
3、Efficiently deliver chimeric antigen receptor (CAR) or T cell receptor (TCR) genes into T cells (CAR-T, TCR-T) or NK cells (CAR-NK) for cancer immunotherapy. Nano-needle transfection offers the potential to enhance engineering efficiency while minimizing impacts on cell viability.
Cells were transfected with GFP DNA using UniquePOKE®, and cell viability and transfection efficiency were assessed 24–48 hours post-transfection.
Cells were transfected with GFP plasmid or mRNA using UniquePOKE®, and cell viability and transfection efficiency were evaluated 24–48 hours post-transfection.